产品货号：
GS2204
中文名称：
BstPAI内切酶
英文名称：
BstPAI
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
High enzyme concentration and incubation at 65℃ for 16hoursresults in star activity.
技术规格
相关搜索：BstPAI内切酶，BstPAI限制性内切酶
使用说明
High enzyme concentration and incubation at 65℃ for 16hoursresults in star activity.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 65℃ in a total reaction volume of 50μl. |
| Recognition sites | GACNN↑NNGTC |
| 来源 | Bacillus stearothermophillus PA |
| Assayed on | Lambda DNA |
| Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 65℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;200 μg/ml BSA;7 mM 2-mercaptoethanol;50%glycerol.Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme less than 5%of the DNA fragments can be ligated |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 25℃ |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | NO |
| Campatible Ends | BoxI,PshAI |
相关搜索：BstPAI内切酶，BstPAI限制性内切酶